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Fig. 6. Silencing of c-subunits of V0 or the F-subunit of V1 causes defects in the cycle of the contractile vacuole complex. (a-d) Control cell, showing normal cycles with filling of the ampullae, fusion with the contractile vacuole and fluid expulsion; (e-h) silencing of any V-ATPase subunit provoked serious perturbation in the series of events and a general prolongation of the cycle, illustrated in this figure by silencing of all c-subunits. Abbreviations: a, ampulla; cv, contractile vacuole; rc, radial canal. Bar, 10 µm.





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