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Fig. 7. EM analysis of the contractile vacuole complex. (a) Control cell compared with one silenced in all c-subunits (b). In cells silenced in any of the c-subunit pairs, the decorated spongiome (cross-sectioned in this figure) is highly disrupted (remaining decorated spongiome is indicated with an arrow) or absent, whereas the smooth spongiome seems to be unaffected. Abbreviations: cc, collecting canal; ds, decorated spongiome; ss, smooth spongiome. Bar, 0.5 µm.