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Fig. 9. Comparison of the exocytotic capacity of wild-type paramecia compared with cells silenced in V-ATPase subunits. The exocytotic capacity of c4, c2/c6 and c2/c4/c6 silenced cells is greatly reduced after 24 hours. Exocytotic capacity was analyzed using the picric acid test and three classes of cells were distinguished: `wild-type' with more than 500 expelled trichocysts, `epsilon' with less than 100, and `minus' with no expelled trichocysts. Data were collected in three different experiments from at least 20 cells per group and the variation was calculated between the experiments.





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