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Fig. 1. Spindle organization during meiosis I-early meiosis II. Projected series of optical sections of oocytes stained with propidium iodide (orange) and an anti-ß-tubulin (green) antibody. (A) Early anaphase I: peripheral MTs intersect at the spindle equator to form discrete clusters (arrowheads) or extend toward the cell cortex (arrow). (B) Late anaphase: the spindle has elongated and the poles appear broad and frayed (arrows), MT clustering at the spindle equator is more pronounced (arrowheads). (B₁) A single optical section of the spindle in B at the level of the equator showing the overlapping of some antiparallel MTs (arrowheads). (C) Telophase: a distinct tubulin accumulation is present midway along the spindle where the opposite MT bundles overlap (arrow). (D) Early prophase II: the peripheral MT network expands and some MTs contact the oocyte surface (small arrowheads); several astral arrays of MTs appear within the equator of the spindle and outside its boundaries (arrows) and a distinct tubulin cluster bulges out the spindle midzone (large arrowhead). (D₁): single optical section of the spindle in D showing that the equatorial tubulin cluster (arrow) is connected by thin bundles to the peripheral MT network (arrowheads). Bars, 2 µm in A-D, D₁ and 0.7 µm in B₁.

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