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Fig. 3. Localization of Asp protein during female meiosis. In all panels MTs are stained green and Asp, orange. (A) Anaphase of meiosis I: Asp is present at the spindle poles. (B) Late anaphase of meiosis I: Asp is localized at the poles and at the spindle equator (arrow). (C) Telophase of meiosis I: Asp localizes in a ring-like structure (arrow) around the accumulation at the central spindle (arrowheads). (D) Early prophase: Asp staining is evident at the spindle poles, at the central spindle, and it is also found at the foci of the sparse asters (arrowheads). (E) Prometaphase and (F) metaphase: Asp remains localized at the outer spindle poles and within the central region of the spindle at the site where the inner poles are forming (arrowheads); a strong labeling is also observed within the individualizing spindles where the central aster is developing (arrow). (G) Anaphase II: Asp accumulates at the spindle poles and at the minus ends of the MTs that organize the central aster (arrow), where a feeble ring-like staining remains. (H) Telophase II: the spindle poles are strongly stained by the anti-Asp antibody, whereas a weak labeling is found within the central aster (arrow). Bar, 3 µm.

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