JCS02439 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Prominin-1-containing particles in the ventricular fluid of the embryonic mouse brain lack cadherin. Double immunofluorescence of a cryosection (20 mm) of an E11.5 mouse embryo using mAb 13A4 against prominin-1 (b,c,e,f, pseudocoloured in red) and either TRITC-phalloidin (0.2 mg/ml; Sigma) (a,c, pseudocoloured in green) or mouse mAb anti-pan-cadherin (1:200; Sigma) (d,f, pseudocoloured in green). Single 5 mm optical sections obtained by confocal microscopy at the level of the glass slide (a-f). (c) A few of the actin-positive particles (which were detected only upon amplification of the original signal) coincide with a small fraction of the prominin-1-positive particles (arrows). (f) Note the lack of cadherin staining. Bar, 10 mm.

• Movie 1 -

  Movie 1. Video clip showing the collection of the ventricular fluid from the neural tube lumen of an E10.5 NMRI mouse embryo.