|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 8. Both the PDZ binding motif and the putative FERM binding motif of CRB3 are critical for the induction of tight junction formation. (A) The indicated cell lines were grown on Transwell filters for 4 days post confluence. Cells were fixed and stained for ZO-1. Images shown are representative fields photographed under 40 x magnification. Arrows indicate small number of tight junctions forming in mycCRB3
ERLI cells (B) Quantification of tight junction formation by different CRB3 mutants. Cells were grown until 4 days post confluent as before, and stained for ZO-1. A tight junction structure was defined as an enclosed ring of smooth, contiguous apical ZO-1 staining. The number of such structures per field was quantified under 20 x magnification as described in Materials and Methods. Graph depicts the calculated mean number±s.e.m. of tight junction structures per field from three independent experiments. (C) Quantification of tight junction formation after 9 days growth past confluence. Tight junction structures were quantified as before. Error bars represent s.e.m. of three independent experiments.
|
