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Fig. 4. Phalloidin, ß-actin and myosin II RLC-GFP colocalise with the structures visualised by transmitted light in the cortical region. (A-C) Rhodamine-phalloidin (1 µM, 30 minutes) labelling of the cortical region overlapped with the peripheral ring visualised with transmitted light scanning microscopy. Images obtained in the transmitted light channel using a green LUT table (A), epifluorescence channel for rhodamine (B) and merged image (C). (D) Chromaffin cell labelled with quinacrine and treated with phalloidin showing static vesicles located over the immobilised cytoplasmic structures observed by transmitted light. (E) Cytoplasmic zone magnified. (F-H) GFP-ß-actin and transmitted light colocalised in the cortical region. Depicted is a cell expressing GFP-ß-actin (F) visualised by confocal microscopy and the corresponding transmitted light image using a red-LUT (G) with the merged image (H). (I-K) Cells overexpressing the double mutant T18A/S19A RLC-GFP were studied by laser confocal microscopy. Depicted is a high magnification field of a cell expressing this inactive form of myosin II, where vesicles are also observed by quinacrine labelling (I). (J) Simultaneous acquisition of transmitted light image corresponding to the same field using a red LUT. (K) Merged fluorescent and transmitted light images. Bar, 10 µm (A-C,D,F-H); 1 µm (E,I-K).





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