|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 7. GFP-ß-actin can be used to study cortical F-actin dynamics in chromaffin cells. Cells infected with amplicons containing the GFP-ß-actin expressing vector were visualised by confocal fluorescence microscopy. (A) Cells expressing GFP-ß-actin were fixed, permeabilised and incubated with phalloidin-rhodamine as indicated in methods. Depicted are confocal images of GFP-ß-actin (A), phalloidin-rhodamine (B) and both channels showing evident overlapping (C). (D) GFP-ß-actin-expressing cell treated with 1 µM latrunculin A for 30 minutes. Images were acquired at 1 Hz, and frames were separated by 5-minute intervals. (E) Fluorescence in the interior or cortical region was determined and plotted against time after latrunculin A addition. Bar, 10 µm.
|
