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Fig. 8. Simultaneous observation of peripheral F-actin reorganisation by GFP-ß-actin fluorescence and transmitted light scanning microscopy. Cells were infected with amplicons containing the GFP-ß-actin construct as indicated in Materials and Methods. GFP fluorescence (A) and transmitted light images (B) of a cortical region were acquired at 1 Hz. Time-lapse high magnification images separated by 10 seconds before (first frame) and after stimulation with 10 µM acetylcholine are shown. Arrows indicate the positions of F-actin disruptions visualised in both channels. (C) Fluorescence determination in three different regions of interest (ROI) located at different distances from the cell limit (boxed areas in A). F-actin transfer to the cell interior follows fragmentation of the peripheral cortex. Bar, 1 µm (A,B).
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