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Fig. 2. Peri-implantation lethality in the absence of PINCH1 expression. (A) Haematoxylin and Eosin staining of sections from E6.5 implantation chambers from heterozygous intercrosses shows that the presumptive mutant chambers contained either degenerated embryos (middle image) or blood cells (right image). d, decidua; epc, ectoplacental cone; ee, extraembryonic ectoderm; e, ectoderm; m, mesoderm. (B) TUNEL staining on sections from E6.5 decidual chambers shows multiple apoptotic cells in the remnants of the PINCH1-null embryo proper (marked region). Control embryos were TUNEL negative. The embryos were identified on consecutive sections stained with DAPI.





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