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Fig. 2. Endogenous MBNL1 colocalizes with CUG and CAG foci in COSM6 cells. (A) Combined FISH/immunofluorescence using Cy5-labeled antisense oligonucleotide probes (Cy5-Oligo), MBNL1 mAb 3A4 (MBNL1) (secondary antibody is Alexa Fluor488) and DAPI staining (DAPI) demonstrating the colocalization of endogenous MBNL1 with CUG or CAG RNA. Magnification x 40. (B) Same as A with magnification x 100. Bars, 10 µM.





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