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Fig. 4. Mlc1 localisation to the Spitzenkörper depends on Spa2 and Bud6. (A,B) spa2{Delta}/spa2{Delta} or bud6{Delta}/bud6{Delta} strains, both expressing Mlc1-YFP, were grown under hyphal-inducing conditions. In both mutants, no spot of Mlc1-YFP is present, but a crescent remains. (C-E) DIC images of mutant hyphal cells (C,D) compared to wild-type hyphal cells (E). In both mutants, the hyphae are broader and more kinked than the wild-type hyphae. Bar, 10 µm.





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