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Fig. 5. Organisation of the Spitzenkörper depends on microtubules. (A) A strain expressing Tub1-YFP was induced to form hyphal germ tubes from unbudded stationary phase yeast cells. Long microtubules are visible along with spots (arrow), which we presume are spindle pole bodies (SPBs). (B) After 60 minutes, 0.1 mg/ml MBC was added and the incubation was continued for a further 10 minutes. Microtubules have disappeared but the SPBs remain (arrow). (C) An Mlc1-YFP expressing strain was also treated with MBC in a parallel experiment. The cells were stained with FM4-64 after 10 minutes of treatment with MBC. The spot of FM4-64 staining has disappeared. (D) Localisation of Mlc1-YFP in the same cells shown in C. The spot of Mlc1-YFP has disappeared but a surface crescent remains in some cells. Bar, 5 µm.





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