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Fig. 2. (A) Schematic illustration of the wild-type and mutant GFP-UCE constructs. The lumenal domain of UCE was replaced with monomeric GFP fused to an N-terminal cleavable preprolactin leader sequence represented by hatched bars. The transmembrane domain of UCE is indicated by shaded bars and the amino acids in the cytoplasmic tail are shown in single letter code. Mutations of the Y⁴⁸⁸ residue and the potential exit signal residues are shown in bold letters. The position of a stop codon is indicated by an asterisk. (B) Schematic illustration of the single and combined mutant GFP-UCE constructs. The constructs have the same organization as those in Fig. 1A. In addition to the Y⁴⁸⁸ and the 502 Stop mutation, residues Q⁴⁹², E⁴⁹³, M⁴⁹⁴ and N⁴⁹⁴ are either singly mutated or mutated simultaneously and the mutant residues are indicated by bold letters.

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