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Fig. 3. High-resolution confocal immunofluorescence images of HeLa cells expressing wild-type or mutant GFP-UCE constructs. HeLa cells were stably transfected with wild-type GFP-UCE (A), GFP-UCE 502 Stop mutant (B) or GFP-UCE Y488A/502 Stop mutant (C). The localization of the constructs was analyzed using high resolution three-dimensional confocal immunofluorescence microscopy followed by projection of z-stacks using the Imaris 4.0 software. Bars, 20 µm.





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