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Fig. 5. High resolution confocal immunofluorescence images of HeLa cells expressing double mutant GFP-UCE constructs of the potential TGN exit signal. HeLa cells were stably transfected with GFP-UCE Y⁴⁸⁸; Q⁴⁹²E-A/502 Stop (A), GFP-UCE Y⁴⁸⁸; M⁴⁹⁴N-A/502 Stop (B), GFP-UCE Y⁴⁸⁸; G⁴⁹⁶E-A/502 Stop (C) or GFP-UCE Y⁴⁸⁸; P⁴⁹⁸L-A/502 Stop (D). Bars, 20 µm.

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