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Fig. 2. Bovine bone osteonectin (B.OSN) `activates' the cell adhesion activity of various extracellular matrix proteins. Attachment was assessed as described in Fig. 1. Matrix proteins + B.OSN (4 µg ml^–1) were mixed together in solution before binding to the plate. These concentrations (µg ml^–1) of matrix proteins [fibronectin (0.1, FN), collagen I (0.005, Col I), laminin (0.4, LM) and vitronectin (0.1, VN)] did not promote or promote a very low level of cell adhesion. Concentrations (µg ml^–1) of human platelet osteonectin (10, H.OSN) and fibronectin (1.25, FN) that do promote cell adhesion were used as controls. Results (mean adhesion ± s.d.) are shown for MDA-231 breast carcinoma cells; similar results were obtained with PC-3 prostate carcinoma cells. **P<0.01 and ***P<0.001 significant difference between matrix protein alone (white bars) and matrix protein + B.OSN (black bars), between H.OSN and FN (1.25 µg ml^–1) and between B.OSN + FN (0.1 µg ml^–1) and FN (1.25 µg ml^–1) was determined by Bonferroni's multiple comparison post-test.

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