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Fig. 7. Effect of bovine serum albumin (BSA) and cytochrome C on `activation' of low levels of fibronectin, laminin, vitronectin and collagen I for cell adhesion. Fibronectin (FN, 0.1 µg ml^–1), laminin (LM, 0.4 µg ml^–1), vitronectin (VN, 0.1 µg ml^–1), collagen I (Col I, 5 ng ml^–1), or no protein (none) were mixed with either no protein (None, white bars) or 4 µg ml^–1 of bovine bone osteonectin (light gray bars), cytochrome C (dark gray bars), or BSA (black bars). Cell adhesion was then assessed as described. Results (mean adhesion ± s.d.) are shown for MDA-231 breast carcinoma cells. *P<0.05, **P<0.05 and ***P<0.001 significant difference between matrix protein alone and matrix protein + osteonectin, cytochrome C or BSA was determined by Bonferroni's multiple comparison post-test after finding a significant difference P<0.0001 among the groups by two-way analysis of variance (ANOVA).

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