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Fig. 1. PLD activation regulates adhesion. (A) At each time point following detachment from substrate (t=0), the number of cells able to adhere to serum-coated plastic over a 5 minute period was measured. (B) At each time point following detachment from substrate (t=0), PtdBut accumulation was quantified over a 5 minute period as a measure of PLD activity in either cells allowed to immediately re-adhere (dotted line) or cells maintained in suspension (solid line). (C) The level of adhesion after 10 or 30 minutes on serum-coated plastic was quantified for cells that had previously been detached from substrate and treated for 5 minutes with 0.5% butan-1-ol/tert-butanol. Results are expressed as a percentage of the number of untreated cells adhered after 10 or 30 minutes. At 10 minutes, the level of untreated cells adhered were approximately 70% of total cells added to each well. At 30 minutes, the level of untreated cells adhered were approximately 90% of total cells added to each well (data not shown). Similar results were obtained in at least three separate experiments and error bars represent the standard deviation from the mean.
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