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Fig. 4. PLD2 stimulates adhesion. (A) Following transfection of GFP-tagged protein constructs, the level of adhesion to serum-coated plastic was measured. The level of adhesion for each individual transfectant was obtained from an average of three experiments and is expressed as a percentage difference to non-transfected cells. (B) The PtdBut accumulation over a 5-minute period was used as a measure of PLD activity of transfected cells following detachment from substrate. PLD1** corresponds to PLD1(H464D/K860E) and PLD2** corresponds to PLD2(H442D/K758E). (C) Following siRNA transfection, the levels of PLD1 mRNA was analysed by quantitative PCR. (D) Following siRNA transfection, the levels of PLD2 mRNA was analysed by quantitative PCR. (E) Following siRNA transfection, the level of PtdBut accumulation over a 5-minute period was quantified as a measure of PLD activity. (F) Following siRNA transfection, the level of adhesion to serum-coated plastic was quantified. Using a Student's t-test, P values of <0.001% were obtained (*) between these samples. Similar results were obtained in at least three separate experiments and error bars represent the standard deviation from the mean.





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