spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. PIPkin I{gamma}b stimulates adhesion. (A) Following transfection with GFP-tagged PIPkin I{alpha} and {gamma} (and derivatives) and the GFP-tagged PH domain for PLC{delta}1, the level of adhesion to serum-coated plastic was quantified and is expressed as a percentage difference to non-transfected cells. Using a Student's t-test, a P value of <0.01% was obtained (*) between these transfectants. (B) The dose-response for increasing levels of PIPkin I{gamma} transfection derived from the total levels observed in (A). `Low', `medium', `high' and `very high' correspond to arbitrary divisions in the level of expression. The levels of expression were determined by the quantity of GFP fluorescence detected by a FACs instrument. The level of adhesion for each individual transfectant was obtained from an average of three experiments and error bars represent the standard deviation from the mean. Using a Student's t-test, a P value of <0.01% was obtained (*) between these transfectants.





Right arrow Return to article