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Fig. 3. Characterization of anti-N-mPres. (a) Immunofluorescence image of a gerbil cochlea stained with anti-N-mPres. The low-magnification image is taken with an epifluorescent microscope with 25x objective. The high-magnification images of OHCs are obtained by confocal microscopy, these are shown in b-e. Immunofluorescence and corresponding phase-contrast images of a normal gerbil (b and d) are compared with material obtained from a prestin knockout mouse (c and e). Both wild-type gerbil and prestin knockout cochlea samples are stained with anti-N-mPres. (f,g) Immunofluorescence and corresponding phase-contrast images of OK cells transiently transfected with wild type, then stained with anti-N-mPres. (h,i) Immunofluorescence and corresponding phase-contrast images of OK cells transiently transfected with the control vector pcDNA3.1-CAT. Bar, 20 µm.





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