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Fig. 4. Representative patterns of wild-type prestin and associated deletion mutants transfected into OK cells. (a) Immunofluorescence and corresponding phase-contrast images of OK cells transiently transfected with cytoplasmic protein EGFP, deletion mutant examples showing types I, and II morphology, and wild type (wt), representing type III morphology. All samples were stained with FITC-labeled anti-N-mPres, except the EGFP preparation. Cell boundaries are marked by arrows. `N' stands for nucleus. Bar, 20 µm. (b) Distribution of the frequency of occurrence of the three cell types for different mutations 48 hours after transfection. The prestin-expressing cells in different samples were classified as types I, II and III, according to the location of prestin (see text); white bars indicate type I, gray bars type II, and black bars type III cells. We note here that some cells classified as type III, found in the Del709 mutant (*), were actually found not to be so after testing for co-localization with the PM marker as described in Fig. 5 (also see text).
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