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Fig. 7. Immunofluorescence images of OK cells transiently transfected with double point mutants: V499G/Y501H and Y520A/Y526A. Red, anti-Na+/K+ ATPase; green, prestin with EGFP attached to its C-terminus. Yellow images are superimposed from green and red images, indicating the co-localization of prestin and Na+/K+ ATPase in the cell's plasma membrane indicated by the arrow. There is no co-localization of prestin with the PM marker for Y520A/Y526A. Images were taken 24 hours after transfection. Bar, 20 µm.





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