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Fig. 3. Clathrin and dynamin do not associate with aberrant early endosomes induced by TSG101 depletion. TSG101-depleted HeLa cells were allowed to internalise Alexa Fluor488-labelled EGF for 3 hours at 37°C. Cells were fixed and examined by fluorescence microscopy for EGF (A,D; green), clathrin (B; red) or dynamin (E; red). Merged images are shown in C and F. Bar, 10 µm.





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