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Fig. 4. Morphology of multicisternal early endosomes in TSG101-depleted cells. TSG101-depleted HeLa cells were incubated with TfHRP for 30 minutes to label early endosomes (A,C), or incubated with EGF and 108 anti-EGFR 10 nm gold for 30 minutes at 4°C, washed, and incubated at 37°C for a further 3 hours, with TfHRP added for the final 30 min (B; note that in this oblique section the inter-cisternal matrix appears as striations). Arrowheads indicate anti-EGFR gold. Arrows indicate matrix. Lamin A-depleted HeLa cells were incubated with EGF and 108 anti-EGFR 10 nm gold for 30 minutes at 4°C, washed, and incubated at 37°C for a further 30 minutes (D) or 3 hours (E). For the final 30 min the cells were incubated with Tf-HRP. Electron dense content; cross linked Tf-HRP. Bars, 0.1 µm (A,D,E); 0.5 µm (B,C).
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