|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Hrs and SNX1 localise to multicisternal endosomes. TSG101-depleted (A,B) and lamin A-depleted (C) HeLa cells were incubated with EGF and 108 anti-EGFR 10 nm gold for 30 minutes at 4°C, washed, and incubated at 37°C for a further 1 hour (for the experiment showing Hrs labelling) or 3 hours (for the experiment showing SNX1), with TfHRP added for the final 30 minutes. The cells were then post-fix labelled with 5 nm gold for Hrs (A) or SNX1 (B, C). Arrowheads indicate anti-EGFR-gold; arrows indicate anti-Hrs-gold or anti-SNX1-gold. Bars, 0.5 µm (A); 0.1 µm (B,C).
|
