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Fig. 6. Lgp120 does not localise to multicisternal endosomes. TSG101-depleted (A) and lamin A-depleted (B) HeLa cells were incubated with EGF and 108 anti-EGFR 10 nm gold for 30 minutes at 4°C, washed, and incubated at 37°C for a further 1 hour, with TfHRP added for the final 30 minutes. The cells were then post-fix labelled with 5 nm gold for lgp120. Arrowheads indicate EGFR-108 gold and arrows indicate matrix. The boxed region in A is shown at a higher magnification in the inset, which has been altered to enhance contrast. Bars, 0.5 µm (A,B) 0.1 µm (inset).
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