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Fig. 8. EGFR and TR colocalise to aberrant early endosomes. TSG101-depleted (A) and lamin A-depleted (B,C) HeLa cells were incubated with EGF and 108 anti-EGFR 10 nm gold for 30 minutes at 4°C, washed, and incubated at 37°C for a further 1 hour, with TfHRP added for the final 30 minutes. The cells were then post-fix labelled with 5 nm gold for TR. Note that B and C are from the same cell labelled for EGFR and TR and show the separate localisations of EGFR (B) and TR (C). EGFR-108 gold complexes are indicated by arrows (10 nm gold). Bars, 0.1 µm.
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