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Fig. 1. Subcellular distribution of GFP, GFP-GAP1IP4BP and GFP-GAP1m in HEK-293 cells under conditions of increased and decreased levels of PtdIns(3,4,5)P3. HEK-293 cells expressing GFP (A), GAP1IP4BP (B) or GAP1m (C) were cultured under conditions designed to increase (i) or decrease (ii) cellular PtdIns(3,4,5)P3. All images represent a 3 µm optical section whose position is indicated by the horizontal white bar (the y axis is in fluorescent units).





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