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Fig. 2. Photobleaching protocol. Fluorescence image series of a HEK-293 cell producing GFP-GAP1IP4BP. (A) Pre-bleaching image (i) shows the cell immediately before the photobleaching. The 3 µm ROI is indicated by the white box. (i) Pre-bleaching. (ii) Immediately after bleaching. (iii) Fluorescence recovery. (B) The fluorescence profile of the photobleaching experiment indicates fluorescence values at pre-bleaching (i) and immediately after bleaching (ii), and the fluorescence recovery (iii). Bar, 6 µm.





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