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Fig. 3. FRAP data for GFP, GFP-GAP1IP4BP and GFP-GAP1m in HEK-293 cells under conditions of increased and decreased levels of PtdIns(3,4,5)P3. Relative mobility (t1/2; A) and mobile fractions (Mf; B) of GFP, GFP-GAP1IP4BP and GFP-GAP1m under conditions designed to increase (white bars) or decrease (black bars) cellular PtdIns(3,4,5)P3 levels. Data are presented as the means±s.e.m. of at least six experiments.





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