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Fig. 1. Localization of the CD36 protein in the adult testis. (A,B) Immunofluorescent labelling of CD36 on cryosection of adult mouse testis. CD36 protein is observed at various locations depending on the stage of differentiation of the seminiferous epithelium. The protein is located at the vicinity of rounded and elongated spermatids in the apical part of the tubules or in the basal Sertoli cytoplasm. Immunostaining of CD36 (A, B1, B2, B3) and nuclei staining with DAPI (B4, B5, B6). Bar, 50 µm. (C) Confocal microscopy analysis of anti-CD36 immunofluorescent staining (green) and nucleus staining (DAPI, blue) in the apical zone of the seminiferous epithelium. Labelling is observed around structures without nuclei, evocative of residual bodies (arrowheads). Bar, 10 µm. (D) Western blot analysis of CD36 in adult mouse testis (9 month). Molecular masses are represented on the left (kDa).





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