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Fig. 4. Subcellular relocalization of CD 36 in 15P-1 cells in presence of germ cells. (A,B) Immunostaining of 15P-1 cells with anti-CD36 antibodies (A1,B1) and corresponding DNA staining with DAPI (B2) merge with the visible image (A2). Germ cell fraction enriched in elongated spermatids (sp, A) or in residual bodies (rb, B) were able to induce the same relocation of CD36 protein along the plasma membrane cytoplasmic extensions of 15P-1 cells (arrowhead). Bar, 10 µm. (C,D) Immunogold localization of CD36 on ultrathin cryosections of 15P-1 cells co-cultured in presence of total germ cells. Each experiment was carried out at least twice. (C) Gold particles present at the plasma membrane invagination; bar, 80 nm. (D) Overview of a spermatid engulfed by 15P-1 cell. Bar, 1.25 µm. Below, enlargement of the previous white square displays the immunogold localization of CD36 within phagocytotic vacuole (arrowhead). Bar, 0.22 µm. (E) Western blot analysis performed using specific antibodies against CD36. Lane 1, round spermatid; lane 2, mixed germ cells; lane 3, spermatocytes; lane 4, syncitium, lane 5, 15P-1 cells. Molecular masses are represented on the left (kDa).

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