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Fig. 5. Morphological changes in 15P-1 cell shape during co-culture experiments. (A) Scanning electron microscopic observation of 15P-1 cells co-cultured in presence of germ cells. Cytoplasmic extension of a Sertoli cell (arrowhead) wrapping around a germ cell (star). Bar, 7 µm. (B) Four most representative slides from a 3D reconstruction obtained by deconvolution microscopic analysis. 15P-1 cells are labelled with CMTMR fluorophore and germ cells are labelled with CMFDA fluorophore (see Materials and Methods). The germ cell (star) interacting with 15P-1 cells has both colours. Bar, 7 µm.





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