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Fig. 8. Fatty acid treatment inhibits the phagocytosis of germ cells but not latex beads. Immunostaining with anti-CD36 antibodies performed on (A) 15P-1 cells (s) co-cultured with germ cells (arrowhead); (B) 15P-1 cells (s) co-cultured with germ cells (arrowhead) in presence of C18:2; (C) 15P-1 cells co-cultured with germ cells and latex beads (arrow) in presence of C18:2 (left). Corresponding field with DNA staining (right). (D) 15P-1 cells were cultured with germ cells (GC) or latex beads (LB) in presence or in absence of fatty acids (FA). After 8 hours co-culture, cells were gently washed with PBS, fixed in 4% PFA and phagocytosed cells or beads were counted. Results were expressed as the proportion (%) of phagocytosed germ cells or latex beads compared with total germ cells or latex beads remaining after washing and fixation. Mean of at least five experiments, bar shows s.e.m. ***: significant variation between GC and GC+FA, P<10–6 (Student's t-test).
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