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Fig. 2. AtSMC1 and AtSMC3 transcript and protein patterns differ in plant tissues. (A) AtSMC1, AtSMC1-3'UTR, AtSMC3 and PTPG expression patterns were examined by RT-PCR analysis of oligo(dT)-generated total cDNA synthesized from RNA isolated from different plant organs: roots, stems, leaves, flower buds. Transcripts for ACT8 were used as a control. RT-PCR products were analyzed by Southern blotting. The number of cycles used for PCR is shown to the right. (B) Relative levels of AtSMC1, AtSMC3 and PTPG transcripts as determined by real-time PCR. Transcript levels were standardized relative to the level of ACT8 cDNA in the sample. (C) AtSMC3 total protein (10 µg) from Arabidopsis root, stem, leaf, flower bud tissues and suspension culture cells was isolated and analyzed by western blotting using anti-AtSMC3 antibody.





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