|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 3. AtSMC3 is present in the cytoplasm, nucleus and nuclear matrix of Arabidopsis suspension cells. (A) Images of anti-AtSMC3 labeling of interphase cells after various extractions and fixing with 4% paraformaldehyde. Cells were sequentially extracted with salt (CSK) followed by detergent extraction buffer to remove soluble cytoplasmic proteins, and subsequently treated with DNase. Untreated cells and buffer without DNAse (mock) were used as controls. DNA was stained with DAPI. AtSMC3 was detected with anti-AtSMC3 antibody followed by treatment with Alexa Fluor 488-labeled secondary antibody. Scale bar: 10 µm. (B) Western blot analysis of AtSMC3 eluted by the series of extractions as described for A. Proteins from these extractions were analyzed by western blotting with anti-AtSMC3 antibody. Cells before treatment were used as the control.
|
