spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Effect of the MTP18 C-terminus on protein localisation and mitochondrial morphology. (A) (left) diagram of myc-tagged (C-terminal red boxes) MTP18 wild-type and mutants that lack the N-terminus (including the predicted TM1) or the C-terminus (including the predicted TM2 and TM3). Transient protein expression was confirmed by western blot (right panel) probed with anti-MTP18 (*, endogenous MTP18; arrow, full-length MTP18–myc; arrowhead, truncated protein variants. (B) Representative confocal immunofluorescence images of transiently transfected COS-7 cells expressing wild-type (upper row), N-terminally truncated (middle row) and C-terminally truncated MTP18 (lower row). Shown is distribution of MTP18 (green, anti-MTP18) and mitochondrial morphology (red, anti-Hsp60). Bars, 20 µm. (C) Immunofluorescence staining of {Delta}91-166-MTP18-myc in transfected COS-7 cells with anti-MTP18 (green) and anti-Hsp60 (red) to investigate its subcellular localisation. Arrowheads, untransfected cells; arrow, cell with low expression levels of {Delta}91-166-MTP18-myc; double-arrow, cell with high expression levels of {Delta}91-166-MTP18-myc.





Right arrow Return to article