QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. MTP18-induced mitochondrial fission is inhibited by counteracting fusion activity. (A) Representative picture of a COS-7 cell transiently expressing MTP18-myc (red) and the mitochondrial adenosine nucleotide transporter as a GFP-fusion protein (ANT-GFP, green). The merged picture shows fragmented mitochondria (yellow). (B) Western blot with extracts from COS-7 cells that had been transiently transfected with MTP18, Drp1 or Mfn1 expression constructs. Endogenous and recombinant proteins were detected with appropriate antibodies. Arrow, MTP18-myc; double arrow, GFP-Mfn fusion protein; arrowheads as labelled. (C) COS-7 cell transfected with dominant negative HA-Drp1^K38A alone (green, anti-Drp1; red, Mitotracker) or together with MTP18-myc (MTP18-myc, red). (D) Representative confocal microscopy images of COS-7 cells transiently transfected with GFP-Mfn1 alone (mitochondrial morphology shown with Mitotracker dye) or together with MTP18-myc, or with GFP-Mfn1^K88T and MTP18-myc. MTP18 was detected with anti-MTP18 (red); GFP-Mfn1 fusion protein is shown in green. (E) Perecentage of transfected cells that exhibit indicated mitochondrial morphologies.

Return to article