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Fig. 1. CI-976 induces tubule formation from all Golgi compartments. Clone 9 rat hepatocytes were treated with solvent control (A,D,G,J,M) or 20 µM CI-976 (B,C,E,F,H,I,K,L, N, O) for various amounts of time as indicated. Cells were then fixed and stained for immunofluorescence localization of various Golgi marker proteins: GPP130 (A-C), ManII (G-I), TGN38 (J-L) and M6PR (M-O). KDEL-R was visualized by transiently transfecting Clone 9 cells with a mutant form of the KDEL-R coupled to GFP (D-F). After 24 hours, the cells were then treated with 20 µM CI-976 and fixed.





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