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Fig. 12. Tf recycling appears to be irreversibly inhibited by CI-976. Clone 9 cells were treated for 10 minutes with DMSO as a solvent control (A,B) or 20 µM CI-976 (C-E). TRITC-Tf was then added for 45 minutes at 37°C. Cells were either fixed (A,C) or were quickly washed in MEM containing 10% Nu-Serum, and subsequently incubated in MEM with 10% Nu-Serum for 1 hour (B,D) or 24 hours (E) at 37°C prior to fixing.





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