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Fig. 7. CI-976 inhibits recycling of Tf. (A-C) Fluorescence micrographs of HeLa cells labeled with TRITC-Tf. (A) Cells incubated with TRITC-Tf for 45 minutes and then fixed. (B) Cells pulse-labeled by TRITC-Tf uptake for 45 minutes followed by a chase without Tf for 25 minutes. (C) Same treatments as B except the chase was in the presence of CI-976 (25 µM). (D) Quantitation of inhibition of Tf recycling in the presence of different concentrations of CI-976; experiments performed as in A-C. Results are expressed as the mean with the error bars representing 1 s.d.





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