Non-canonical YXXG endocytic motifs: recognition by AP2 and preferential utilization in P2X4 receptors

JCS02451 Supplementary Material

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• Supplemental Figure 1 -

  Fig. S1. Decreasing the distance from the plasma membrane to the motifs has little effect on motif recognition. (A) Representative confocal images of CD8-4CD chimeras following live-labeling as described in Materials and Methods. (B) Histogram, showing the proportion of protein detected at the cell surface (red) or in internal compartments (green) after labeling (n=35-57). To allow comparison with the histogram in Fig. 3, these data were normalized to the values obtained when CD8-4C and CD8-4CD were tested in parallel. Bar, 10 mm, **, P<0.01.