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Fig. 1. Localization of NPP1 and NPP2 in HEK293 cells. (A) Cells were transiently transfected with HA-NPP1-Myc and NPP2-EGFP (upper three panels) or with NPP2-EGFP alone (lower nine panels). After 24 hours, the cells were fixed with 2% formaldehyde. NPP2-EGFP, HA-NPP1-Myc, the ER-marker BiP, the Golgi-marker Golgin-97 and the trans-Golgi network marker TGN38 were visualized by confocal microscopy using the spontaneous green fluorescence of EGFP, anti-Myc antibodies, anti-BiP antibodies, anti-TGN38 antibodies or anti-Golgin-97 antibodies, as indicated. The right panels represent the merges of the left and middle panels. (B) HEK293 cells were transiently transfected with HA-NPP1-Myc or HA-NPP2-Myc. At the indicated time points, the fusion proteins were detected by immunoblotting of the cell lysates and the culture medium with anti-Myc antibodies.





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