QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Effect of siRNA specific for LAMP1 and/or LAMP2 on cell death induced by serum and amino acid depletion. Cells transfected with the indicated siRNAs (24 hours) were cultured in complete medium (CM) or nutrient-free (NF) medium for 24 hours, followed by staining with propidium iodine (PI) and DiOC₆(3) for determination of viability and the mitochondrial transmembrane potential, respectively. Representative FACS pictograms are shown in A. Numbers refer to the percentage of cells in each quadrant. Note that PI⁺ cells tend to be DiOC₆(3)^low, meaning that the former can be represented as a subpopulation of the latter. Results are means (±s.d.) of three independent experiments. The percentage of DiOC₆(3)^low cells and PI⁺ cells obtained in response to a variety of different siRNAs are shown in B and C, respectively. An electron microscopic picture of a representative cell subjected to LAMP2 siRNA (64) and nutrient depletion (20 hours) is shown in D. N, nucleus; AV, antophagic vacuole; M, mitochondrion.

Return to article