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Fig. 7. Contribution of caspases to vacuolization-associated death. (A,B) Activation of caspase-3 induced by a combination of LAMP-specific siRNA and starvation. Cells were treated as indicated and stained with an antibody recognizing activated caspase-3. Representative fluorescence microphotographs are shown in A and quantitative data (X±s.d., n=3) are shown in B. Bar, 10 µm. (C,D) Effect of the pan-caspase inhibitor Z-VAD-fmk. Cells were subjected to the indicated LAMP1 and/or LAMP2 knockdown and then cultured in the absence or presence of nutrients and/or Z-VAD-fmk for 24 hours. Then, the loss of the 
m (C) and viability (D) was measured with the indicated dyes, as in Fig. 2.
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