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Fig. 2. Clathrin pit internalization in N-WASPdel/del cells. (A,B) Images show representative examples of CCS (red in merged images) internalizing with (B) or without (A) detectable actin (green in merged images) assembly. In these cells, the majority of pit-like structures (72.1%, see also Fig. 1D) disappeared without detectable actin recruitment (A). The remaining pits (B) recruited actin in a manner that was indistinguishable in intensity to parental control cells (C). Time is given in seconds; scale bar: 2 µm. (C) Columns represent average peak fluorescence intensities shortly before internalization of the components indicated, which were assessed in those CCS recruiting actin in both cells types. For neither component, significant differences between the two cell types could be scored.





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