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Fig. 5. N-WASP deficiency causes a reduction of EGF internalization. (A) After 3 hours of starvation, cells were incubated with rhodamine-EGF at 37°C for 10 minutes and directly processed for fluorescence microscopy. Red, rhodamine-EGF; blue, DAPI nuclear staining. (B) After 3 hours of starvation, cells were incubated with 1.5 ng/ml of ¹²⁵I-EGF at 37°C for the time points indicated. The amount of surface and internalized radioactivity was determined at the end of the incubation. After correction for non-specific binding, the rate of internalization was expressed as the ratio between internalized/surface radioactivity (mean ± s.e.m. from three independent experiments). The differences were confirmed to be statistically significant (P<0.032 by linear regression analysis).

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